Facility Control and Maintenance

Mice intended for export are housed in our barrier facilities. Access to the facilities are strictly limited to trained laboratory and animal care personnel by key card access. Personal protective equipment (PPE) is required for entry into the facility which includes disposable gowns, shoe covers, gloves, hair bonnet and masks. Animal care personnel are dedicated to the facility and/or room. Animals are housed and maintained in positive-pressure, individually ventilated cages/units (IVC/U) which are changed every 1-2 weeks. Mice are provided with corncob bedding and paper nest enrichment and receive irradiated feed. All caging, bedding and enrichment are autoclaved prior to use. Processed water (reverse osmosis, chlorinated) is supplied by an Edstrom automatic watering system. Animals leaving the barrier cannot return to the facility.

 

Health Status

The health status of all colonies is monitored quarterly by sampling from sentinel mice on a biweekly basis after exposure to soiled bedding. The pooled samples of soiled bedding are obtained from cages on their respective racks. The sentinel mice are 6-8 weeks of age and housed two per cage on each side of a rack unit, which contains 70/80 cages. Retired breeders are also tested as part of routine monitoring. The results are reported as positive tests/total test performed. Health monitoring includes necropsy, serologic testing, bacteriology, endo/ecto parasitology, histopathology, and DNA analysis. The tested items include all microbes included in the national microbial test standard of laboratory animals.

 

Importing Animals

For imports, only specific pathogen free (SPF) animals are accepted and must be rederived and tested prior to release into the barrier. These animals are quarantined in isolators, and samples (including hair, feces or serology samples) of the received mice are submitted to GemPharmatech (GPT) microbial testing center. Animals can only be transported to the breeding animal rooms by cesarean section or embryo transfer once passing microbial tests.

 

VIRUSES

Method

Ectromelia virus (Ect) ELISA
Mouse hepatitis virus (MHV) ELISA
Sendai virus (SV) ELISA
Pneumonia virus of mice (PVM) ELISA
Reovirus 3 (Reo-3) ELISA
Minute virus of mice (MVM) ELISA
Lymphocytic choriomeningitis (LCMV) ELISA
Hantavirus (HV) ELISA
Theiler's Mouse Encephalomyelitis Virus (TMEV) ELISA
Mouse Adenovirus (FL) (MAD 1) ELISA
Mouse Adenovirus (K87) (MAD 2) ELISA
Polyoma Virus (POLY) ELISA
Mouse Parvovirus (MPV) ELISA
Mouse Cytomegalovirus (MCMV) ELISA
Murine Norovirus (MNV) ELISA
Epizootic Diarrhea of Infant Mice (EDIM) ELISA
K virus ELISA
LDH elevating virus (LDV) ELISA
Mouse Kidney Parvovirus (MKPV) PCR
   

BACTERIA & MYCOPLASMA

 

Helicobacter Culture
Mycoplasma pulmonis ELISA
Corynebacterium kutscheri Culture
Tyzzer's organism ELISA
Pasteurella pneumotropica Culture
Klebsiella pneumoniae Culture
Staphylococcus aureus Culture
Pseudomonas aeruginosa Culture
Pathogenic dermal fungi PCR
Yersinia pseudotuberculosis PCR
Yesinia enterocolitica PCR
Streptobacillus moniliformis Culture
Bordetella bronchiseptica Culture
Escherichia coli O115 a,C,K (B) Culture
Streptococcus pneumoniae Culture
β-hemolyticstreptococcus Culture
Helicobacter PCR
Cilia-Associated Respiratory Bacillus (CARB) ELISA
Corynebacterium bovis Culture
   

PARASITES & PROTOZOA

 

Ectoparasites, e.g., fleas, mites, and lice Micro
Toxoplasma gondii ELISA
Helminth, e.g., pinworms Micro
Flagellata Micro
Ciliata Micro
Encephalitozoon cunicul ELISA
Pneumocystis spp. PCR
   

OTHER

 

Klebsiella oxytoca Culture
Pasteurella multocida Culture
Citrobacter rodentium Culture
Proteus mirabilis Culture

 

Additionally, GemPharmatech partners with several respected institutions and organizations to breed and house mice. For information on the health monitoring and testing practices of these facilities, please contact us.

GemPharmatech Health Monitoring Program — GemPharmatech