Cytometric Bead Array

Molecular Platform
PK/PD Platform
Pathological Diagnosis Platform
Cell-based Service Platform
Cell Line Engineering In vitro Pharmacological EvaluationTumor-bearing Mouse Models
Flow Cytometry Platform
Services and AdvantagesImmune Cell ProfilingTumor Infiltrating Lymphocyte (TILs) DetectionRed Blood Cell and Platelet DetectionTissue Sample AnalysisAntibody Binding AssayCytometric Bead Array
Imaging and Analysis Platform
Behavioral Analysis Platform

The Cytometric Bead Array (CBA) is a multiplex protein quantification method based on the flow cytometry system, enabling the simultaneous detection of multiple markers within a single sample. It utilizes microspheres with different fluorescent intensities, each coated with specific antibodies (capture antibodies) that can recognize particular proteins. These microspheres interact with the sample (such as serum, plasma, culture supernatant, cell lysate, etc.) and with detection antibodies labeled with PE. The resulting complex is then analyzed by a flow cytometer. By comparing the PE fluorescence intensity to a standard curve generated through software analysis and reference standards, the CBA assay can perform quantification analysis of specific proteins. Compared to ELISA, the CBA assay is more sample-saving and convenient to operate.


Experimental Case Study: Detection of TNF-α and IL-6 levels in mouse serum before and after drug treatment.


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Advantages


  • Proficient in detecting a variety of cytokines, with the capability to detect 6-8 cytokines in a single sample simultaneously. This method is not only sample-saving but also highly sensitive, with a detection range in the picogram level.

  • Offerings include fixed panels or customized cytokine combinations.

 


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